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Stimulation of Lactic Acid Bacteria by Substances Concomitant to Certain Proteolytic Enzymes¹

Department of Animal Industry, N. C. State College, Raleigh

ABSTRACT

Enzymatic hydrolyzate of lactalbumin possessed growth-stimulatory ability for certain lactic acid bacteria when added to milk and to a nutritionally complete semi-synthetic medium. The stimulation afforded by this hydrolyzate has been shown to originate in the enzyme preparation used. The enzyme itself was not stimulatory, as evidenced by the fact that impure trypsin preparations were stimulatory but crystalline trypsin was not. The stimulatory factor(s) associated with impure preparations of certain enzymes must be considered in any study of "strepogenin" activity of protein hydrolyzates prepared with such enzymes.

¹ Published with the approval of the Director of Research, North Carolina Agricultural Experiment Station, Raleigh, as Paper No. 588 of the Journal Series.