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Chemical Estimation of Progesterone in the Blood of Cattle, Sheep, and Goats¹

Department of Dairy Husbandry, University of Missouri, Columbia

ABSTRACT

During the course of studies on the hormonal control of mammary gland growth, there arose a need for estimations of the progesterone secretion rate. This was true especially in the larger domesticated animals, in which urinary metabolites of the hormone have received little attention (4). Furthermore, the significance of pregnandiol determinations is at least uncertain in species in which it is excreted (11, 21) and its absence in the urine of goats, cattle, and horses (1, 17) prevents even pregnandiol estimation. Estimation of the progesterone secretion rate by replacement therapy in pregnant cattle, after ablation of the corpus luteum, provides another technique of indirect measurement (12, 18). However, by this technique placental progesterone secretion is not determined. Within recent years more direct methods have been sought. Hooker and Forbes (9) developed a biological method which showed relatively high concentrations of progesterone in the blood. Edgar (6) described a chemical method of assay of blood progesterone.

¹ Contribution from the Mo. Agr. Expt. Station, Journal Series No. 1494. Approved by the Director.