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A Semi-Automatic Apparatus for Maintaining Solvents with Changing Polarity in Chromatographic Columns

Department of Food Technology, University of Illinois

ABSTRACT

A number of adaptations have been reported for maintaining a constant flow of a solvent with changing polarity in chromatographic columns (1, 2, 3, 4). During our studies on the identification of organic acids formed during the ripening of cheese it was felt necessary to design an apparatus that would provide a flow of increasing concentration of butanol in chloroform under pressure and at the same time maintain a constant liquid level above the chromatographic column.

Figure 1 gives the details of such an apparatus. The top reservoir is a 500-ml. flask (A) connected to a 100-ml. receptacle (B) through a standard ball and socket joint (E) by a 1-mm. capillary (D) and a 4-mm. tube (C). C open into a funnel in A above the 250-ml. level an reaches below the 50-ml. level in B. Capillary D provides the flow of the solvent from A to B which is automatically cut off when the tip of the tube C becomes immersed under the solvent.