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Observations on the Acetate and Citrate Metabolism of Streptococcus Lactis and Streptococcus Cremoris¹

Department of Animal Industry, North Carolina State College, Raleigh

ABSTRACT

A study of the acetate-citrate metabolism of the lactic group of streptococci revealed that strains of Streptococcus cremoris responded significantly when a level of 10 mg. per milliliter of acetate was used in the medium but failed to respond when citrate was added at the same concentration; with some strains this level of citrate proved to be inhibitory. Streptococcus lactis responded significantly to this concentration of either acetate or citrate; the magnitude of the response to citrate was significantly greater than to acetate. These data support the contention that two distinct species exist in the lactic group of streptococci.

Experiments with calcium pantothenate revealed that all of the strains tested were unable to grow in its absence. Furthermore, coenzyme A as a source of pantothenate was considerably less effective in meeting this requirement than was Ca pantothenate.

¹ Published with the approval of the Director of Research, North Carolina Agricultural Experiment Station, as Paper No. 583 of the Journal Series.