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Diluters for Bovine Semen. II. Effect of Milk Proteins upon Spermatozoan Livability¹

Dairy Cattle Breeding Research Center, The Pennsylvania State University, State College

ABSTRACT

Since Thacker and Almquist (22) showed that heated milk maintains satisfactory motility and fertility of bovine spermatozoa, heated fresh homogenized milk or skimmilk has been shown by these workers (23) and Dreher and Webb (4) to give fertility results equivalent to, or better than, those obtained with the conventional yolk-buffer diluters. However, studies with reconstituted nonfat dry milk solids, canned skimmilk, and evaporated whole milk have not yielded as encouraging results to date. Marion and Olson (16) and Bennett (2) found that nonfat dry milk solids prepared at low temperatures gave extremely poor results unless heated after reconstitution, whereas reconstituted high temperature powders, without additional heating, gave results more nearly approaching those obtained with heated fluid milk and yolk-citrate. Jacquet and Cassou (9) have questioned the use of canned skimmilk after observing wide variations in fertility between bulls. Collins (3) obtained fair livability but poor fertility results with semen diluted in evaporated milk reconstituted with an equal volume of distilled water.

¹ Published with the approval of The Pennsylvania Agricultural Experiment Station as Journal Article No. 1814.

² Present address: Carnation Milk Farm, Carnation, Washington.