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Diluters for Bovine Semen. V. A Comparison of Heated Milk and Egg Yolk-Citrate as Diluters for Semen from Bulls of High and Low Fertility¹

Dairy Cattle Breeding Research Center, The Pennsylvania State University, State College

ABSTRACT

Heated homogenized milk has been shown to provide fertility results equal to or better than egg yolk-buffer diluters (1, 3, 4, 6), and there is evidence that it is of special value as a diluent for semen from bulls of relatively low fertility. Almquist and Thacker (2) and Dreher and Webb (3) have reported significant increases in the nonreturn rates of relatively infertile bulls when their semen was diluted with heated homogenized milk containing penicillin and streptomycin as compared to egg yolk diluters with antibiotics.

The objectives of the fertility tests reported herein were: (a) to study the effect of adding penicillin and streptomycin to heated milk diluter, and (b) to compare heated milk and egg yolk-citrate as diluters for semen from bulls of relatively high and low fertility.

EXPERIMENTAL PROCEDURE

Two split sample experiments were conducted at the Western Pennsylvania Artificial Breeding Cooperative from July 23 to October 16, 1951, and from January 6 to February 28, 1953. In each study the bulls were selected and classified as being either high or low in fertility on yolk-citrate diluter prior to the start of the trial.

¹ Published with the approval of The Pennsylvania Agricultural Experiment Station as Journal Article No. 1880.