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Some Nutritional Requirements of Bovine Rumen Bacteria¹

Departments of Bacteriology and Dairy Husbandry, University of Maryland, College Park

ABSTRACT

During the course of nutritional investigations of bacteria from the bovine rumen, it has become evident that rumen fluid is an indispensable constituent of any medium used to cultivate maximum total numbers of these bacteria. The rumen fluid requirements of pure cultures of rumen bacteria have been noted by Hungate (10) and Bryant (2) and indirectly by Burroughs et al. (4). In this laboratory, Doetsch et al. (5) also have reported that bacteria cannot be cultured from higher dilutions of normal rumen fluid if rumen fluid is omitted from a standard medium used for making counts. The purpose of this work was to obtain information that would reveal the nature of the essentiality of rumen fluid. Such information, although concerned more directly with the metabolism and physiology of the bacteria within the rumen, would also have important implications with respect to the nutrition of the animal itself, since cow and bacteria are so intimately related.

¹ Paper No. A-431, Contribution No. 2475 of the Maryland Agricultural Experiment Station.