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Animal Husbandry, Agricultural Chemistry, and Dairy Husbandry Departments University of Missouri, Columbia
ABSTRACT
Since Phillips (16) and Phillips and Lardy (17) recommended egg yolk as a constituent of media for the dilution and storage of spermatozoa, evidence has accumulated which demonstrates that egg yolk is a necessary requirement for maximum survival and the maintenance of the fertilizing capacity of these cells. The complex nature of egg yolk, however, made it difficult to ascertain the specific factors responsible for the beneficial effects and the manner in which they were producing these effects.
One of the important functions of egg yolk in a diluting medium was described in a series of papers by: Lasley et al. (9), Easley et al. (5), Lasley and Bogart (11), Lasley and Mayer (12), aud Bogart and Mayer (2). In these papers it was suggested that egg yolk is important in protecting the spermatozoa from adverse environmental conditions. Mayer and Lasley (14) were able to isolate from egg yolk a small fraction, soluble in phosphate buffer, which would protect a higher proportion of the spermatozoa from temperature shock than would whole egg yolk.
1 Missouri Agricultural Experiment Station, Journal Series No. 1347.
2 Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy.
3 This investigation was supported (in part) by a research grant from the National Institutes of Health, Public Health Service, and from Armour Research Laboratories, Chicago.
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