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The Lipase of Pseudomonas fragi¹ II. Factors Affecting Lipase Production

Dairy Industry Section, Iowa Agricultural Experiment Station, Ames

ABSTRACT

Quantitative studies on lipase production and the factors affecting the liberation of the enzyme in growth media by Pseudomonas fragi have not been reported. This study was undertaken to determine the effect of certain physical, physico-chemical, and nutritional factors upon lipase production by this organism.

METHODS

The cultures used in this study were obtained from the stock culture collection of the Dairy Bacteriology Laboratories at Iowa State College. Stock cultures were carried on tryptone-glucose-beef extract agar slants which were stored at 2 to 3° C. and were transferred at monthly intervals. Before inoculating test media, the cultures were transferred twice in broth at 21° C. for 24 hours; one drop of the second transfer was inoculated into the growth medium, which was incubated at 15° C. for 3 days unless otherwise stated. The nutrient broth used contained 0.5 g. peptone, 0.3 g. beef extract, 10 ml. 0.5 M KH₂PO₄, enough 0.1 N NaOH, to give the desired pH and distilled water to a total volume of 100 ml.

¹ Journal Paper No. J-2129 of the Iowa Agricultural Experiment Station, Ames, Iowa. Project No. 1050.