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Dilution of Bull Semen with Frozen Egg Yolk-Sodium Citrate¹

South Carolina Agricultural Experiment Station, Clemson

ABSTRACT

By the use of a split-sample technique, the fertility of bovine semen was studied using four diluters: (a) freshly prepared control diluter, C; (b) freshly prepared diluter to which penicillin-streptomycin had beeen added, PS; (c) diluter prepared and frozen one month prior to use, FC; and (d) diluter to which penicillin-streptomycin had been added and then frozen one month prior to use, FPS.

Results, based on 60- to 90-day non-returns to 1,558 first services were as follows: C, 60.5 per cent; PS, 58.3 per cent; FC, 61.8 per cent; and FPS, 63.3 per cent. An analysis of variance showed that there were no significant differences in conception rates.

¹ Technical contribution No. 205, South Carolina Agricultural Experiment Station, Clemson.