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Diluters for Bovine Semen. I. Fertility and Motility of Bovine Spermatozoa in Boiled Milk¹

Dairy Cattle Breeding Research Center, Pennsylvania Agricultural Experiment Station, State College

ABSTRACT

Although much attention has been given to the improvement of semen diluters during the past decade, the egg yolk-buffer diluter developed by Phillips and Lardy (5) in 1940 still provides the basic medium for diluting bull spermatozoa in routine artificial breeding operations. They tested the livability of bull spermatozoa in evaporated milk also and were able to obtain fair motility survival for 48 hours, but after 72 hours all of the spermatozoa were immotile. In 1942 Underbjerg et al. (7) compared undiluted semen to semen diluted with autoclaved milk or egg yolk-phosphate and concluded that the diluters had little beneficial effect on fertility. The first promising report concerning the possible use of boiled cow's milk as a diluting fluid for bull semen came from Czechoslovakia in 1950. Michajilov (3) suggested that fresh, clean milk from a healthy cow might be used as a diluter for bull semen after it had been boiled, filtered, and cooled.

¹ Authorized for publication August 27, 1952, as paper No. 1756 in the Journal Series of the Pennsylvania Agricultural Experiment Station.

² The data contained in this paper are part of a thesis submitted by the senior author to the Graduate School of The Pennsylvania State College in partial fulfillment of the requirements for the degree of Master of Science; June, 1952.

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