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Journal of Dairy Science Vol. 36 No. 2 143-151
© 1953 by American Dairy Science Association ®
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Nutritional Factors Influencing Growth and Lipase Production by Geotrichum Candidum

W. O. Nelson

Laboratory of Bacteriology, Dairy Science Department, University of Illinois, Urbana

ABSTRACT

The conditions necessary for optimum microbial lipase activity vary with the source of the enzyme, the substrate, and the treatment accorded the lipase preparation. Similarly, it appears that the production of bacterial and fungal lipases is related directly to the nutritional and physical environment of the cultures under observation. Thus, it has been suggested (1) that certain bacterial lipases are adaptive in nature and that production is stimulated by the presence of specific substrates, or that the elaboration of the enzyme depends upon preferential utilization of certain substrates by the organisms. Peters and Nelson (3) observed with Candida lipolytica that low lipase activity was associated with conditions favorable for rapid growth, whereas high lipase activity was associated with less favorable conditions. In this connection, Tammisto (9) suggested that the failure of certain lipolytic species to hydrolize fat when they were grown on glycerol-fat agar was due to the inactivation of the lipase by acids developed in the glycerol fermentation.







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Copyright © 1953 by the American Dairy Science Association ®.