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Separation of Sodium, Potassium, Magnesium, and Calcium in Milk Ash by Ion-Exchange Chromatography

The Department of Agricultural Biochemistry and The Institute of Nutrition and Food Technology, The Ohio State University, Columbus

ABSTRACT

A simple method is described for the separation of sodium, potassium, magnesium, and calcium in salt mixtures, milk ash, etc., by ion-exchange chromatography. After the solution containing the sample on the ion-exchange column is chromatographed, the elution volume ranges for each constituent element are determined in a preliminary trial. The constituent elements then are eluted as chlorides by suitable concentrations of HC1, collecting the eluate volume fractions for the respective cations in the volume ranges found in the preliminary study. Aliquots of each fraction are evaporated to dryness to expel uncombined HC1 and then taken up with distilled water. The amount of cation-bound chloride then is determined by the Mohr titration with 0.02 N AgNO₃.