HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Collins, R. A. Articles by Bomstein, R. A. Search for Related Content PubMed Articles by Collins, R. A. Articles by Bomstein, R. A.

Further Studies on the Folic Acid and Vitamin B₁₂ Content of Cow's Milk¹

Mrs. R. A. Bomstein

Department of Biochemistry, College of Agriculture, University of Wisconsin, Madison

ABSTRACT

The relic acid and vitamin B₁₂ content of cow's milk has been reported by Collins, Harper, Schreiber and Elvehjem (4). The colostrum from one Guernsey cow was observed to contain higher levels of these vitamins than the milk; therefore, further analyses have been made in an effort to determine the average relic acid and vitamin B12 content of the colostrum of the various breeds of dairy cows. Since the data reported by Collins et al. (4) represent samples obtained only during the summer of 1950, the content of these vitamins in cow's milk during other seasons also was investigated.

Experiment and Result

The relic acid and vitamin B₁₂content of the milk samples was determined microbiologically using the methods described by Collins et al. (4). The vitamin B12 activity was measured using Lactobacillus leichmarnnii ATCC (4797), and the relic acid activity was measured using Streptococcus faecalis R. The free vitamin activity was determined acidimetrically by adding the diluted whole milk samples directly to the assay tubes.

¹ Published with the approval of the Director of the Wisconsin Agricultural Experiment Station. This work was supported in part by funds granted by the National Dairy Council, Chicago, on behalf of the American Dairy Association.

² Present address: International Minerals and Chemical Corp., Central Research Lab., 5401 Harrison St., Skokie, Ill.