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Riboflavin Studies with Calves. I. Preparation of a Riboflavin-Deficient Milk^1,2

Departments of Dairy Husbandry and Agricultural Chemistry, Purdue University, Lafayette, Indiana

ABSTRACT

A low-riboflavin potency milk suitable for experimental feeding was obtained either by photolysis with ultra-violet light or by chromatography with Florisil.

Chromatography removed from normal milk about 90 per cent of the riboflavin and 85 per cent of the thiamine, yielding a product containing about 0.12 and 0.053 mg. per liter, respectively. Other constituents and properties, including flavor, apparently were unaffected by chromatography.

The low-potency milk yielded a value of 0.22 mg. riboflavin per liter when assayed by ordinary microbiological procedures and 0.034 mg. per liter when the acetone extract was assayed photofluorometrically; however, a more precise value of 0.12 mg. riboflavin per liter was obtained when the modifications described here in were employed.

¹ Journal paper no. 601 of Purdue University Agricultural Experiment Station.

² Data presented in this paper are taken from a thesis submitted by E. G. Moody in partial fulfillment of requirements for a Ph.D. degree, Purdue University, Lafayette, 1951.

³ Present address: Agriculture Department, Arizona State College, Tempe.