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Separation of -, ß- and -Casein¹

Eastern Regional Research Laboratory², Philadelphia 18, Penn.

ABSTRACT

Two methods are described for separation of the electrophoretic components of casein. In the first method, the separation is accomplished by precipitation from 50 per cent alcohol solutions of casein by means of variations in temperature, pH and ionic strength and by isoelectric precipitation from water.

The second method is based on the solubility of the casein components in aqueous urea. The urea method is relatively simple and gives products with the same composition and properties as those obtained by the pH and also the 50 per cent alcohol methods.

¹ Presented in part at the 118th American Chemical Society Meeting, Chicago, Ill., Sept., 1950. Abstracts of papers, p. 16C.

² One of the laboratories of the Bureau of Agricultural and Industrial Chemistry, Agricultural Research Administration, U.S.D.A.

This article has been cited by other articles:

				N.J. Hipp, M.L. Groves, and T.L. McMeekin Tensile Properties of Bristle Fibers Made from {alpha}- and {beta}-Casein Textile Research Journal, July 1, 1954; 24(7): 618 - 623. [Abstract] [PDF]