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Vitamin B₁₂ in Blood of Newborn and Colostrum-Fed Calves and in Colostrum and Normal Milk of Holstein and Jersey Cows^1,2

Agricultural and Mechanical College of Texas and Texas Agricultural Experiment Station, College Station

ABSTRACT

Vitamin B₁₂ determinations were made on the blood of calves before ingestion of colostrum and subsequently to 8 days of age. No significant differences were observed in relation to age (within this range), breed or the food ingested.

Holstein colostrum showed nearly double the values observed on Jersey colostrum. The colostrum B₁₂ values for both breeds declined gradually. The Jersey milk, showing averages of 7.0 millimicrograms after the 15th day, appeared to have declined to "herd average" levels. The Holstein milk maintained a slightly higher value through the 45th day.

Forty-eight Jersey cows fresh from 6 to 351 days showed an average vitamin B₁₂ value of 7.6 millimicrograms per milliliter. Morning milk averaged 8.7 versus 6.9 for evening milk (with significance at the 0.05 level).

¹ Supported in part by Project RC 1862, Division of Research Grants and Fellowships, U. S. Public Health Service, National Institute of Health, Bethesda, Maryland.

² The crystalline vitamin B₁₂ used in the microbiological assays was generously supplied by D. F. Green, Veterinary Department, Merck and Co., Rahway, New Jersey.

³ Department of Dairy Husbandry.

⁴ Biochemistry and Nutrition and Poultry Husbandry Departments.