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The Fertility of Bull Semen Extended with Yolk-Citrate Containing Varying Proportions of Egg Yolk¹

Kentucky Agricultural Experiment Station, Lexington

Marshall C. Carpenter

Kentucky Artificial Breeding Association

ABSTRACT

During the months of June and July, 1950, 182 semen samples from 26 bulls were split into two equal portions and extended with yolk-citrate prepared to contain egg yolk and sodium citrate solution in proportions of 1 : 1, 1 : 3 or 1 : 5. Semen in each of the extenders was used to breed 1,200 to 1,400 cows. There was no significant difference between the 1 : 1 and the 1 : 3 series. However, the semen extended in 1 : 5 yolk-citrate had a fertility of 7.8 percentage units lower than that extended in 1 : 3 yolk-citrate. This difference, when tested by Chisquare, was highly significant.

¹ The investigation reported in this paper is in connection with a project of the Kentucky Agricultural Experiment Station and is published by permission of the director.