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The Fertility of Bovine Semen in Citrate-Yolk Extenders Containing Added Catalase

Department of Dairy Science, University of Illinois, Urbana

R. W. Bratton and R. H. Foote

Laboratory of Animal Breeding and Artificial Insemination Department of Animal Husbandry, Cornell University, Ithaca, N. Y.

ABSTRACT

Using the split sample technique, the spermatozoan livability and the fertility of 47 semen samples from 24 Holstein bulls were studied when extended to contain approximately 11.9 x 10⁶ motile spermatozoa per milliliter in citrate-yolk and citrate-sulfanilamide-yolk extenders with and without added catalase.

On the basis of the average per cent 60- to 90-day non-returns to service to approximately 1,700 first and second service cows per treatment, the extenders compared as follows: 3.6 citrate-yolk without added catalase, 61.4; 3.6 citrate-yolk with added catalase, 60.7; 3.6 citrate-sulfanilamide-yolk without added catalase, 62.2; and 3.6 citrate-sulfanilamide-yolk with added catalase, 61.7.

Differences in spermatozoan livability and fertility in the various extenders were not significant.