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Journal of Dairy Science Vol. 33 No. 6 442-448
© 1950 by American Dairy Science Association ®
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Live Spermatozoa Relationships and Fertility of Dairy Bull Semen1

Edward J. Stone2, James E. Johnston and John P. Mixner

New Jersey Agricultural Experiment Station, Sussex

ABSTRACT

The technique of differential staining of live and dead spermatozoa was utilized in this study to determine the relationship of the initial percentage of live spermatozoa in semen samples, as well as the percentage of spermatozoa in diluted semen surviving a 0° C. temperature for 10 min., to other semen characters and fertility. Data on 236 semen samples from 19 bulls, including 81 samples from 14 bulls on which fertility data were based, were used in this study. An analysis of variance of the data for the various semen characters indicated that highly significant differences existed among the bulls in regard to these semen characters. A correlation analysis was presented on a "total," "between" and "within bull" basis, but only the analysis on a "between bull" basis had coefficients of correlation sufficiently high to have any prediction value. A multiple coefficient of correlation of +0.69* of fertility with spermatozoa concentration and the percentage of spermatozoa surviving a 0° C. cold shock was obtained. A partial regression equation involving these factors was presented.

The authors wish to acknowledge the generous assistance given by Robert E. Mather in outlining the statistical procedures used in the analysis of the data.

FOOTNOTES

1 Paper of the Journal Series, New Jersey Agricultural Experiment Station, Rutgers University-the State University of New Jersey, Deparment of Dairy Industry.

2 Now at the Dept. of Dairy Ind., North Dakota Agr. College, Fargo.






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Copyright © 1950 by the American Dairy Science Association ®.