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Fertility and Motility of Bovine Spermatozoa in Buffered Whole Egg Extenders

Laboratory of Animal Breeding and Artificial Insemination Department of Animal Husbandry, Cornell University, Ithaca, New York

ABSTRACT

By means of the split sample technique, comparisons were made between the standard 2.9 and 3.6 per cent citrate-sulfanilamide-yolk extenders and whole egg extenders composed of three parts of either a 1.9 or a 2.9 per cent citrate buffer, containing 400 mg. per cent of succinylsulfathiazole and one part of blended liquid whole egg (yolk + albumen).

On the basis of approximately 11,000 first- and second-service cows inseminated artificially with each of these two types of extenders, the 60- to 90-day non-returns averaged 62.3 per cent for the citrate-sulfanilamide-yolk formulae and 61.6 per cent for the citrate-succinylsulfathiazole-whole egg formulae. Spermatozoan motility was satisfactory in both types of extenders.

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