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Motility of Bovine Spermatozoa in Buffered Whole Egg

Laboratory of Animal Breeding and Artificial Insemination, Department of Animal Husbandry, Cornell University, Ithaca, New York

ABSTRACT

Spermatozoan motility at 5° C. was as satisfactory in extenders composed of three parts of a 2.9 per cent sodium citrate dihydrate buffer containing 0.4 per cent of succinylsulfathiazole or 0.4 per cent succinylsulfanilamide and one part of liquid whole egg as in the standard 2.9 per cent citrate-sulfanilamide-yolk extender.

The costs of the whole egg extenders were approximately 25 per cent of that of the yolk extender. The former could be prepared more quickly and glassware coming in contact with them was much easier to clean. The spermatozoa were more visible and more active in the whole egg extenders, thereby facilitating microscopic examinations.