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Extraction and Isolation of Gamma Globulin from the Bovine Thymus Gland¹

Department of Dairy Husbandry, University of Missouri, Columbia

ABSTRACT

1. Fresh bovine thymus glands, acetone-dried and ether-defatted thymus tissue, and desiccated calf thymus tissue were extracted with 1 M saline solution. No gamma globulin could be precipitated from these extracts either by adjusting the pH to 7.7 and ethanol concentration to 18 per cent by volume at - 10° C., or by ammonium sulphate at 34 per cent saturation at pH 6.
   
2. Fresh bovine thymus glands were washed three times with physiological saline and lysed with one volume of distilled water and then extracted with one volume of 20 per cent saline. The saline extract yielded 60 mg. of acetone-dried gamma globulin on 34 per cent saturation with ammonium sulphate at pH 6.
   
3. It is concluded that the bovine thymus, though containing small amounts of gamma globulin, cannot be used as a rich source of immune globulin.
   

¹ Contribution from the Department of Dairy Husbandry, Missouri Agricultural Experiment Station, Journal Series no. 1172.