HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lo, H. K. Articles by Shaw, J. C. Search for Related Content PubMed Articles by Lo, H. K. Articles by Shaw, J. C.

The Validity of the Allen Volumetric Procedure for the Determination of Blood Lipids of Cows on Different Feeding Regimes^1,2

Department of Dairy Husbandry, Maryland Agricultural Experiment Station, College Park

ABSTRACT

In a previous paper by Chung et al. (1), a comparison was made between a modified Allen blood fat procedure and a fractionation procedure based on the extraction of blood lipids. This study indicated that the Allen procedure was satisfactory under normal conditions for the determination of the plasma lipid fraction composed of the lipids other than phospholipids. However, it was deemed advisable to obtain additional data to establish the relationships more specifically and especially to determine the value of the procedure under varying feeding regimes. This report deals with the validity of a modification of the Allen volumetric method for the determination of blood lipids of cows on different levels of energy and fat intake.

EXPERIMENTAL PROCEDURE

For this study, blood samples were drawn from cows which had been fed on different levels of fat and energy intake in connection with another project which was under way simultaneously. These rations resulted in marked variations in the plasma lipids.

¹ Paper no. A282, Contribution no. 2228 of the Maryland Agricultural Experiment Station.

² This work was supported in part by grants from the Quaker Oats Co. and Procter and Gamble Co.

² The experimental data in this paper are taken in part from a thesis presented by H. K. Lo in partial fulfillment of the requirement for the degree of Master of Science in Dairy Husbandry, University of Maryland.