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A Comparison of the Allen Volumetric Blood Fat Procedure with an Extraction Procedure¹,^,1

Department of Dairy Husbandry, Maryland Agricultural Experiment Station, College Park

ABSTRACT

Conclusions: The fractionation of the Allen lipid column showed that only small amounts of phospholipids were present. A comparison of these fractions with fractions obtained by extraction of the same plasma gave similar values, indicating that the lipids other than phospholipids are separated rather completely by the Allen procedure. The lipids other than phospholipids were 83.1 ± 1.41 per cent of the Allen lipid value. The latter was 63.3 ± 1.17 per cent of the total plasma lipids. The correlation between the Allen lipid value and the value for plasma lipids other than phospholipids was high (r = 0.866 ± 0.047).

¹ Paper no. A281, Contribution no. 2227 of the Maryland Agricultural Experiment Station.

² This work was supported in part by grants from the Quaker Oats Co. and Procter and Gamble Co.

³ The experimental data in this paper are taken in part from a thesis presented by A. C. Chung in partial fulfillment of the requirements for the degree of Master of Science in Dairy Husbandry, University of Maryland.