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The Determination of Protein Sulfhydryl Groups with Iodine and O-Iodosobenzoate by an Amperometric Titration.¹

Division of Agricultural Biochemistry, University of Minnesota, St. Paul 1

ABSTRACT

An amperometric adaptation of the "dead stop" titration technique has been applied to determine the sulfhydryl groups of proteins with o-iodosobenzoate and iodine. As applied to native proteins, the oxidation is largely produced by the iodine liberated in the course of determining the excess o-iodosobenzoate.

The method appears to be specific and quantitative for sulfhydryl groups, since any very reactive groups which might be overoxidized by iodine react first with the o-iodosobenzoate.

¹ Paper no. 2538, Scientific Journal Series, Minnesota Agricultural Experiment Station, St. Paul 1.

² The data in this paper are to be included in a thesis to be submitted by Bruce L. Larson to the University of Minnesota in partial fulfillment of the requirements for the Ph.D. degree. This investigation was supported in part by a research grant from the American Dry Milk Institute, Inc.