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Resazurin Reducing Time as an Indicator of Bovine Semen Fertilizing Capacity¹

Department of Dairy Husbandry, State College of Washington, Pullman

ABSTRACT

Resazurin was tested as a possible indicator of fertilizing capacity. The test for reduction time was made by using 11 mg. of resazurin dye in 200 ml. of distilled water. One-tenth ml. of this solution was added to 0.2 ml. of undiluted semen and incubated at 45° C. Time required for reduction to pink, the first endpoint, and reduction further to white was recorded for 924 semen samples from 45 bulls representing the Guernsey, Jersey and Holstein breeds. The relationship of the pink and white endpoints to survival at 3.3 and 45° C. (94 semen samples), initial motility (564 semen samples), concentration (558 semen samples) and methylene blue reduction time (376 semen samples) was high. The time required to reduce to pink in unincubated semen showed a highly significant correlation of –0.141 to non-return rate on 371 semen samples which were used for 20 or more first and second services. The white endpoint showed a slightly higher correlation of –0.169, as compared with –0.151 for the pink endpoint when the semen was incubated for 30 min. at 45° C. before making the test.

¹ Published as Scientific paper no. 925, Washington Agricultural Experiment Stations, Institute of Agricultural Sciences, State College of Washington, Pullman.

² Acknowledgment is made to D. E. Waldo, Manager of Northwest Co-op. Breeders, Mt. Vernon, Washington, and E. T. Coie, Manager of Evergreen Breeders Association, Chehalis, Washington, for making this study possible; and to Louis Mikota for making routine laboratory tests at Northwest Co-op. Breeders.

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