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Motility of Bovine Spermatozoa and Control of Bacteria at 5 and 25° C. in Extenders Containing Sulfanilamide, Penicillin, Streptomycin and Polymyxin

Laboratory of Animal Breeding and Artificial Insemination, Department of Animal Husbandry, Cornell University, Ithaca, N. Y.

ABSTRACT

The feasibility of storing bovine semen at 25° C. for use in artificial insemination to eliminate the expense of refrigerating the semen at 5° C. was investigated. Sulfanilamide, penicillin, streptomycin, polymyxin and a combination of these were added to 3.6 per cent citrate-yolk extender. The citrate-yolk extender containing no sulfanilamide or antibiotics served as the control. Eighteen semen samples were stored in each of the six extenders at 5° C. and at 25° C. The per cent of motile spermatozoa after 24 hr. of storage was lower when the semen was stored at 25° C. than when it was stored at 5° C. except in the extender containing the combination of antibacterial agents. In nearly all samples, this combination of sulfanilamide and antibiotics completely inhibited bacterial growth at both temperatures. This combination of antibacterial agents gives promise of making possible the development of an extender for bovine semen which will not require refrigeration.