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The Adaptation of a Standard Curve to the Turbidometric Method of Assay of Hyaluronidase in Bull Semen¹

New Jersey Agricultural Experiment Station, Sussex

ABSTRACT

In using the turbidometric assay for hyaluronidase, considerable flexibility in the calculation of hyluronidase potency in semen is achieved by reference to a standard preparation of hyaluronidase. The coefficient of correlation between mg. of standard hyaluronidase and colorimeter meter reading was –0.985 ± 0.006.

Hyaluronidase assay values of semen in terms of mg. of standard hyaluronidase, obtained by diluting semen at various rates, conformed to the standard regression line of purified hyaluronidase.

A coefficient of correlation of +0.954 ± 0.008 was obtained between turbidity reducing units and mg. of standard hyaluronidase equivalent for 117 semen samples, indicating the essential sameness of the two measures of hyaluronidase potency.

The results indicate the validity of expressing semen hyaluronidase potencies in terms of milligrams of a standard hyaluronidase preparation.

¹ Paper of the journal series, New Jersey Agricultural Experiment Station, Rutgers University—The State University of New Jersey, Department of Dairy Industry.