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Milk Lipase System. III. Further Studies of the Solvent Extraction Procedure for Obtaining Fat from Milk for Titration¹

Maryland Agricultural Experiment Station, College Park

ABSTRACT

1. Results are presented of two attempts to modify the solvent extraction procedure for the obtaining of fat in lipase studies so as to increase the efficiency of recovery of the lower fatty acids. The two modifications studied were: (a) the saturation of the milk with salts (MgS0₄ and NaCl) before extraction and (b) the acidification of the milk to pH 2 with H₂SO₄ before extraction.
   
2. Of these modifications, only the acid adjustment of the milk or cream was found to yield sufficient improvement in recovery as to warrant its adoption as a part of the solvent extraction procedure.
   
3. A comparison of the solvent extraction method and a procedure involving continuous extraction of milk previously dried in plaster of Paris reveals that the latter method does not remove efficiently the free fatty acids from milk.
   

¹ Scientific paper no. A225. Contribution no. 2150 of the Maryland Agricultural Experiment Station (Dairy Department).

² Present address: Dairy Technology Dept., Ohio State University, Columbus.