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The Fertility of Bovine Semen Cooled with and without the Addition of Citrate-Sulfanilamide-Yolk Extender¹

Laboratory of Animal Breeding and Artificial Insemination, Department of Animal Husbandry, Cornell University, Ithaca, New York

ABSTRACT

Sixty-four ejaculates of bovine semen were divided and cooled from 30° C. to 5° C. in 75 minutes with and without the addition of citrate-sulfanilamide-yolk extender prior to cooling.

Based on 60- to 90-day non-returns to 8,518 first and second service cows, the fertility level of the pre-extended semen (semen cooled in extender) was 59.3 per cent and that of the post-extended semen (cooled without extender), 52.8 per cent. The difference between treatments of 6.5 percentage units was highly-significant statistically.

Motility estimates made after 3, 24, 48, 72 and 96 hr. of storage indicated that the samples cooled without extender had a definitely lower percentage of motile spermatozoa. However, by using covariance analysis the higher per cent non-returns for the pre-extended semen could not be accounted for on the basis of more motile spermatozoa per insemination.

¹ In view of the improved livability of spermatozoa in present day media the authors consider the word "extender" more appropriate than "diluter" for describing these media, and therefore have adopted the word "extender" in this report.

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