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The Relationship of the Change in pH Effected by Incubation to Other Semen Characteristics¹

New Jersey Agricultural Experiment Station, Sussex

ABSTRACT

1. A study was made of the relationship of the decrease in pH during incubation of 203 semen specimens composed of 473 ejaculates from 11 bulls to other semen characteristics.
   
2. Highly significant coefficients of correlation found between the change in pH and other characteristics of semen were: concentration of spermatozoa, 0.46; initial motility of spermatozoa, 0.44; viability of spermatozoa, 0.21; and percentage of morphologically abnormal spermatozoa, –0.32. The multiple coefficient of correlation (0.57) between these four semen characteristics and the drop in pH was highly significant. These data suggest a significant relationship of the pH change in incubated semen to a number of different semen characteristics and indicate its value as an in direct measure of over-all semen metabolism.
   
3. The results of this study, in combination with those of other in vestigations involving fertility data, would seem to indicate that the change in pH effected by incubation is probably the best simple, quick test of semen quality available at the present time.
   

¹ Paper of the Journal Series, New Jersey Agricultural Experiment Station, Rutgers University, Department of Dairy Industry.

² Present address, Cornell University, Ithaca, New York.