Journal of Dairy Science Vol. 30 No. 4 245-253
© 1947 by American Dairy Science Association ®
The Stabilization of Carotene in Dehydrated Legumes (Alfalfa) and Cereal Grasses1,2
A. W. Halverson and
E. B. Hart
Department of Biochemistry, College of Agriculture, University of Wisconsin, Madison
ABSTRACT
- Destruction of the lipoxidase by autoclaving for 1 hour at 15 lbs. pressure did not preserve the carotene content of dehydrated alfalfa or cereal grasses exposed to air at room temperature.
- The addition of 5 to 10 per cent of water to these dehydrated materials and then lightly packing them in receptacles sealed with flexo wax or Durex wax preserved the carotene completely for 3 months, when held at room temperature (22–25° C). The total water content for the oats was 15–20 percent and for the alfalfa 12–17 percent.
- The process of preservation appears to be a restoration of more rapid respiratory enzyme action with utilization of the oxygen and formation of carbon dioxide. There was no indication that bacterial action directly was responsible for the changes that occurred. Temperature changes during the process of respiration were negligible in the masses of material used.
- It is evident that receptacles with minimum or no air and CO2 effusion rates are necessary for success in the preservation of the carotene by the method outlined. Oxygen must be excluded or at least held at a low concentration. This phase of the problem is under further study.
FOOTNOTES
1 Published with the approval of the Director of the Wisconsin Agricultural Experiment Station.
2 This work supported in part by a grant from the Wisconsin Alumni Research Foundation
Copyright © 1947 by the American Dairy Science Association ®.