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Department of Dairy Industry, Cornell University, Ithaca, New York
ABSTRACT
According to Hammersten1 "perfectly fresh amphoteric milk does not coagulate on boiling but forms a pellicle consisting of casein and lime-salts which rapidly reforms after being removed. Even after passing a current of carbon dioxide through the fresh milk it does not coagulate on boiling. In proportion as the formation of lactic acid advances this behavior changes and soon a stage is reached when the milk, which has previously had carbonic acid passed through it, coagulates on boiling. At a second stage it coagulates alone on heating, thus it coagulates by passing carbon dioxide alone without boiling; and lastly when the formation of lactic acid is sufficient, it coagulates spontaneously at the ordinary temperature forming a solid mass. It may also happen especially in the warmth, that the casein clot contracts and a yellowish or yellowish green acid liquid (acid whey) separates."
According to Van Slyke, "when milk is treated with acid or acid salts the casein is precipitated as a heavy, white solid in more or less flocculent form, depending on conditions of treatment.
1 A Text Book of Physiological Chemistry. Hammerstein and Mandel From Allen's Commercial Organic Analysis, vol. viii, p. 121.
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