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From the Department of Biochemistry, College of Agriculture, University of Wisconsin, Madison
ABSTRACT
The data presented suggest the following pertinent facts in relation to the storage of bull semen.
The addition of blood-fibrin, or arginine, were without measureable effect upon motility of sperm. Glucose additions in concentrations of 0.02 to 0.04 M improved in most cases the storage of bull semen environed in egg yolk-buffer. Freshly purified lecithin, or lipositol from crude soybean lecithin free of free fats or fatty acids with 0.04 M glucose, or with a mixture of 0.02 M galactose and 0.04 M glucose provided a highly effective pabulum for the storage of the sperm. In many experiments a combination of galactose and glucose (conc. indicated above) was superior to glucose alone. Inositol in 0.05 to 0.1 M solutions and in combination with glucose and galactose likewise benefited sperm motility. The addition of certain gums was beneficial while gum damar and gum guaiac were definitely toxic. Certain antibiotics as well as certain of the sulfonamides were effective in controlling bacterial growth without impairment of sperm survival.
On the basis of these data a synthetic pabulum for bull sperm storage may be used. Its essential ingredients should include freshly purified lipids, specific sugars—glucose and galactose, a buffer system, a gum to supply the proper physical consistency and an agent to control bacterial contamination.
1 Published with the approval of the Director of the Wisconsin Agricultural Experiment Station.
The streptomycin used in this study was kindly supplied by Merck and Company, through the courtesy of Dr. Green.
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