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Further Studies on the Stabilization of Carotene in Dehydrated Alfalfa and Cereal Grasses

From the Department of Biochemistry, University of Wisconsin, Madison

ABSTRACT

SUPPLEMENTARY NOTE^*

More recent experiments in our laboratory show that the carotene of alfalfa and "cerophyl" is stable when subjected to autoclaving at 15 pounds pressure for one hour, when complete displacement of the air by steam vapor is maintained. This initial treatment destroys the carotene oxidase and what destruction of carotene follows on storage is due to "autocatalysis" in the presence of oxygen. On autoclaving the chlorophyll is destroyed and the material becomes gray in color.

After autoclaving in the absence of air and drying both the alfalfa and cerophyl were stored in the dark at room temperature for three months. Samples were also made into pellets 1/2 by 1/2 inch and others into larger pellets 4 by 3 inches. Some of the pellets were dipped in flaxo-wax. The alfalfa left loose lost 50% of its carotene in three months, the cerophyl 56%. The small or large pellets of alfalfa lost in the same time about 36%, the cerophyl about 56%.

^* This note supplements the report which appears in the January 1945 issue pages 1–14.—EDITOR.