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The Influence of pH on the Attraction between the Fat Globules and Leucocytes of Milk^*

Michigan Agricultural Experimental Station

ABSTRACT

Lowering the pH of a washed-cream, heat-treated-whey mixture containing added washed leucocytes from pH 6.5 to 1.5 resulted in marked differences in the attraction between the fat and the leucocytes. Migration of the washed leucocytes with the rising fat globules was greatest at pH 4.3, the isoelectric point of the fat globules. Below this value the attraction between the fat globules and the leucocytes was not as great as when the pH was. above that of the isoelectric point. Thus, when the electric charge of the fat globules was reversed from negative to positive, the washed leucocytes did not respond so readily to the "sweeping" action of the fat.

The results indicate that the attraction of the fat globules and the washed leucocytes is based, in part at least, upon the opposite electric charges carried by the fat globules and the leucocytes found in milk.

^* Journal Article No. 730 (n.s.), Michigan Agricultural Experiment Station.