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The Methylene-Blue Reduction Test and its Relation to Other Measures of Quality in Bull Semen

Department of Animal Husbandry, Cornell University, Ithaca, New York

ABSTRACT

The relationship of the methylene-blue test to other criteria of measuring semen quality is shown by correlation coefficients. In the samples of this study the methylene-blue test was not significantly correlated with the proportion of morphologically abnormal spermatozoa, nor with ascorbic acid content or the initial glucose level of fresh semen, nor with the glucose loss in diluted semen after 10 days' storage at 5° C.

Statistically highly significant correlations were shown between the methylene-blue reduction time and volume of the ejaculate, spermatozoa count, initial spermatozoa motility, initial pH, and the initial lactic acid level of fresh semen. Equally significant associations were shown in diluted semen with the methylene-blue reduction time by glucose loss, by lactic acid gain, and by livability after an hour's incubation at 46.5° C., and by lactic acid gain and by livability after 10 days' storage at 5° C.

The methylene-blue reduction test, along with spermatozoa count and initial motility estimates, is recommended for routine prediction of semen quality.