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Action of Penicillin against Mastitis Organisms in Milk

Storrs Agricultural Experiment Station, University of Connecticut, Storrs, Conn.

ABSTRACT

1. A comparative in vitro study of the action of penicillin against the common mastitis organisms suspended in sterile milk and broth indicated that milk, at pH 7.0, is as favorable a medium for penicillin action as buffered beef infusion broth.
   
2. Arranged as follows in order of decreasing resistance to the action of penicillin, Str. agalactiae and Str. uberis, S. aureus and Str. viridans, Str. dysgalactiae and C. pyogenes and the hemolytic group C streptococcus were successfully rendered nonviable in vitro in milk with small amounts of penicillin.
   
3. A strain of E. coli isolated from an acute case of mastitis was refractory to penicillin in concentrations up to 99 units.
   
4. Strains within a species were susceptible in most instances to approximately the same concentrations of penicillin.
   

^* It is a pleasure to acknowledge the technical assistance of Miss Helen P. Pearson.