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A Practical Method of Coloring Semen for Identification Purposes

Department of Biochemistry, College of Agriculture, University of Wisconsin, Madison

ABSTRACT

In the operational aspects of artificial insemination of dairy cattle not infrequently the wrong sample of semen is used. This most frequently occurs when the semen of one breed is used to breed cows of another breed. This may not be serious in the grade herd but it may be serious in the purebred herd. A means of reducing this type of operational error would be of practical value.

Since certain vital dyes are apparently tolerated by the animal body without apparent harm a series of stains and dyes were tested for their effect upon bull spermatozoa in storage.

METHODS

The method used in these observations was the storage time of active sperm in yolk-buffer (1) in comparison with yolk buffer plus a suitable and sufficient concentration of a dye to distinctly color the diluent. Fresh semen was diluted 1:5 with the fresh egg yolk buffer prepared after the method of Phillips and Lardy (1).