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A Technique for the Simultaneous Measurement of Semen Quality and Testis Histology in Vitamin A Studies of the Dairy Bull^*

Purdue University Agricultural Experiment Station, Lafayette, Ind.

ABSTRACT

Vitamin A deficiency is the most common of the known vitamin deficiencies which occur in cattle. The clinical symptoms, gross and microscopic pathology and therapy of vitamin A deficiency have been reported by many investigators and are not reviewed in this report.

It has long been known that vitamin A deficiency results in the cessation of spermatogenesis and atrophy of the testis in laboratory animals (8, 9) and in cattle recent studies (3, 4, 5, 6, 7) have shown vitamin A deficiency to have a similar effect on the testis of the bull.

When small numbers of animals are available it is obviously necessary to obtain as much information from each individual as possible. The standard procedures which have been developed for the measurement of semen quality are of unquestioned value but it is especially desirable to be able to determine the effects of vitamin A deficiency and therapy upon the spermatogenic tissue of the testis itself.

^* Journal paper No. 153, Purdue University Agricultural Experiment Station.