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Rumen Synthesis of the Vitamin B Complex as Influenced by Ration Composition^*

From the Department of Biochemistry, College of Agriculture, University of Wisconsin, Madison

ABSTRACT

The addition of urea as a source of nitrogen definitely increased the synthesis of riboflavin, nicotinic acid, biotin and pantothenic acid in the bovine rumen when a readily available carbohydrate was present. Pyridoxine and "folic acid" could not be too closely correlated with ration composition. In the absence of a readily fermentable carbohydrate and probably a low population of microorganisms the synthesis of the members of the B complex is not at a maximum.

The data indicate that thiamine may not be synthesized in the rumen. However, it seems more than probable that it is synthesized, but absorbed or destroyed at a rate greater than its synthesis.

^* Published with the approval of the Director of the Wisconsin Agricultural Experiment Station. This work was supported in part by a grant from E. I. DuPont de Nemours and Co., Inc.

Thanks are extended to Mr. T. D. Luckey and Miss Jean Collord for the "folic acid" and pyridoxine assay respectively.