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The Pennsylvania Agricultural Experiment Station, State College, Pennsylvania
ABSTRACT
The ascorbic acid content of evaporated milk has been investigated by Meulemans and de Haas (9), who concluded that the canned product contains as much vitamin C as does "prepared" fresh milk. These workers apparently used a direct titration procedure with 2,6-dichlorobenzenoneindophenol and obtained data ranging from 20 to 30 mg. per liter of ascorbic acid in evaporated milk reconstituted to a fluid basis.
Henry, Houston, Kon and Osborne (3), using the precipitation technique of Kon and Watson (8), titrated milk serum with the indophenol dye and found losses of about 30 per cent in the total ascorbic acid content of evaporated milk due to manufacture, with a subsequent gradual loss in storage. Appreciable quantities of dehydroascorbic acid were noted upon reduction of the milk serum with hydrogen sulfide, scrubbing with nitrogen and subsequent titration.
Woessner, Elvehjem and Schuette (13) found only insignificant amounts of dehydroascorbic acid in evaporated milk using a photoelectric method and extrapolating galvanometer readings to zero time, which procedure they found corrected errors due to spurious reducing substances resulting from the effect of hydrogen sulfide on milk ingredients other than dehydroascorbic acid.
* Authorized for publication April 8, 1943, as paper No. 1175 in the Journal Series of the Pennsylvania Agricultural Station
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