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A Comparative Study of the Frost Little Plate and Standard Plate Methods for the Bacteriological Examination of Milk, Cream, and Ice Cream^*

Agricultural Experiment Station, East Lansing, Michigan

B. L. Gilbert and G. J. Turney

Department of Health, Lansing, Michigan

ABSTRACT

The incubation of Frost little plates at 37° C. in a moist chamber for four hours yielded bacteria colony counts of milk and cream comparable to the standard plate count. The chocolate particles of some chocolate milk may confuse the technician unless a 1 to 10 or 1 to 100 dilution is made before preparing the little plate.

Comparable counts were obtained upon examination of ice cream by the standard plate and Frost little plate methods when the little plates were incubated for a minimum period of eight hours at 37° C. in a moist chamber. Ice cream containing seeds, nuts, or chocolate particles may be diluted as in the case of chocolate milk before preparing the little plates.

^* Editor's Note: Because of the present limited supply of agar for bacteriological purposes this report is of particular timely interest.