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A New Colorimetric Method for the Determination of Free Fatty Acids in Milk Fat

Department of Dairy Industry, New York State College of Agriculture, Cornell University, Ithaca, N. Y.

ABSTRACT

A simple and quick colorimetric method for the determination of free fatty acids in milk fat is described. The method consists in dissolving 1 ml. of the milk fat in 3 ml. of a saturated solution of the base of neutral red in xylol and comparing with a set of standards of known oleic acid contents. The method is shown to be highly sensitive and accurate. In neutral fat and in xylol the dye base gives an orange yellow solution. Free fatty acids form red soaps with the dye base and the degree of shift to the red is proportional to the concentration of soap and, therefore, of the free fatty acids. Equal normal concentrations of various fatty acids produce an equal shift in the color.