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The Effect of Amphyl on Motility and Longevity of Bovine Spermatozoa^*

New Jersey Agricultural Experiment Station, New Brunswick, N. J.

ABSTRACT

The motility of bovine sperm in undiluted semen ceased within 30 seconds after the addition of 0.5 per cent Amphyl, a disinfectant containing synthetic organic compounds.

Indications are that sperm, suspended in an egg yolk-phosphate buffer dilution medium containing from 0.2 to 0.5 per cent of Amphyl, maintained motility longer than that of sperm suspended in the same medium but containing no Amphyl.

Sperm suspended in phosphate buffer solution containing one per cent Amphyl soon lost all motility.

Sperm suspended in egg yolk-phosphate buffer solution containing Lysol lost all motility within three days, whereas, those suspended in the same medium but containing Zephiran remained motile for a period equal to that of the control sample.

Hemolytic staphylococci were completely destroyed in phosphate buffer and in egg yolk solution containing one per cent Amphyl; many of the staphylococci continued to grow after seven days when suspended in egg yolk-phosphate buffer solution containing one per cent Amphyl.

^* Journal series paper of the New Jersey Agricultural Experiment Station, Rutgers University, Department of Dairy Husbandry.