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Normal Variations in the Amount of Ascorbic Acid in the Blood of Dairy Cattle^*

Dairy Husbandry Section and the Chemical Section of the Michigan Agricultural Experiment Station, East Lansing

ABSTRACT

1. Nineteen calves, 4 heifers and 24 cows were used to determine the variations that occur in the plasma ascorbic acid of normal dairy animals.
   
2. The mean ascorbic acid value found for the calves was 0.32 mg. per 100 ml. of plasma (range 0.03 to 0.77 mg.), for the heifers was 0.49 mg. (range 0.24 to 0.80 mg.) and for the cows was 0.44 mg. (range 0.11 to 0.80 mg.).
   
3. Marked variations were found to occur in the amount of ascorbic acid in the blood when the samples were taken at the same hour each day for a period of 8 or 10 days.
   
4. Marked variations were also found to occur in the amount of ascorbic acid in the blood of individual cows when the samples were obtained at 3-hour intervals.
   
5. These data indicate that random ascorbic acid determinations are of little value in determining the normal concentration of this vitamin in the blood of dairy animals.
   

^* Published with the approval of the Director of the Agricultural Experiment Station as Journal Article no. 588 (n.s.).