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Butterfat and Silage Carotenoids^*

Department of Biochemistry, College of Agriculture, University of Wisconsin, Madison

ABSTRACT

Besides carotene and xanthophyll, pigments formed by the action of acids in silage are carried over into the butter fat of the milk. The usual methods of carotene analysis on butter fat do not distinguish between carotene and non-carotene pigments. By chromatographic methods the distribution of pigments in butters from cows on different forages was found to be as follows: pasture, 80 per cent carotene, 20 per cent non-carotene; molasses alfalfa silage, 68 per cent carotene, 32 per cent non-carotene; phosphoric acid alfalfa silage, 65 per cent carotene, 35 per cent non-carotene.

^* Published with the approval of the Director of the Wisconsin Agricultural Experiment Station.