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The Determination of Fat in the Presence of Free Fatty Acids. II. Differences in the Behavior of Individual Acids in the Mojoinnier Test

Brooklyn College, Brooklyn, New York
Cornell University, Ithaca, New York

ABSTRACT

The Mojonnier test does not recover equal percentages of the various free acids which may be present in the butterfat. This is due, in part at least, to variations in the amount of different acids volatilized when the sample is heated to remove the ether.

It seems probable that the recovery of free acids by the Mojonnier method can be traced to hydrolysis of the ammonium soaps with the subsequent extraction of the liberated acid. The degree of hydrolysis would depend upon the nature of the acid, but it might be reduced in all cases by the substitution of a stronger base for the ammonia. Lithium hydroxide offers some promise, but its saponifying action must be studied more carefully before it can be recommended.

^* Contribution 34 from the Department of Biology.