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Storage of Dairy Bull Spermatozoa^*

University of Missouri, Columbia, Mo.

ABSTRACT

An appreciation of the limitations and possibilities of storing dairy bull semen and retaining its fertility is necessary in order to define the practical limits of artificial insemination with shipped or stored semen. While various diluents have been proposed and different storage temperatures have been suggested, there still remain many unexplained factors concerning the successful storage of dairy bull semen. One difficulty in semen storage research has been the lack of an accurate criterion of fertility. Since the time of survival with vigorous motility of bull spermatozoa stored undiluted at 40° F. has been shown to be correlated with the fertility of bull semen (14), this property of the semen may be used in evaluating storage methods more accurately than simple determination of time of survival.

Refrigeration has been recognized for some time as the most effective method of preserving bull semen (15). Spermatozoa normally live only a short while at body temperature, and room temperature (70° F.) has likewise been found too high for successful semen storage (17).

^* Contributed by the Department of Dairy Husbandry, Missouri College of Agriculture, Journal Series No. 729.